TY - JOUR AU - Sagar, Vasanthkumar AU - HS Kumar, Arun PY - 2020/05/21 Y2 - 2024/03/29 TI - Efficacy of Natural Compounds from Tinospora cordifolia against SARS-CoV-2 Protease, Surface Glycoprotein and RNA Polymerase JF - Biology, Engineering, Medicine and Science Reports JA - BEMS Reports VL - 6 IS - 1 SE - Original Research Article DO - 10.5530/bems.6.1.2 UR - https://bemsreports.org/index.php/bems/article/view/102 SP - 06-08 AB - <p style="text-align: justify;"><strong>Background: </strong>Antiviral activity of natural compounds from <em>Tinospora cordifolia</em> (Amritaballi) were evaluated for their efficacy against SARS-CoV-2 targets involved in virus attachment and replication. <strong>Materials and Methods: </strong>The binding efficacy (binding affinity, Ki and IC<sub>50 </sub>values) of natural compounds from <em>Tinospora cordifolia</em> were tested using<em> in silco </em>tools against four key SARS-CoV-2 targets i.e., 1) surface glycoprotein (6VSB) and 2) Receptor binding domain (6M0J) both responsible for attachment of the virus to host cell, 3)RNA dependent RNA polymerase (6M71) and 4) main protease (6Y84) responsible for replication of the virus in the host cell.<strong> Results: </strong>Berberine, Isocolumbin, Magnoflorine and Tinocordiside showed high binding efficacy against all the four key SARS-CoV-2 targets. Tinocordiside and Isocolumbin showed IC<sub>50</sub> value of &lt; 1μM against both 6Y84 and 6VSB. <strong>Conclusion: </strong>At least four natural compounds from <em>Tinospora cordifolia</em> showed high binding efficacy against SARS-CoV-2 targets involved in attachment and replication of the virus. Hence validating the merit of using <em>Tinospora cordifolia</em> in the clinical management of infection caused by SARS-CoV-2.</p> ER -